Our light microscopy capabilities provide a range of imaging solutions ideal for imaging biological samples, such as live or fixed cells and tissue sections. We provide support and advice spanning custom sample preparation (including sample preparation equipment, validated reagents and consumables) through to sophisticated 3D image data analysis.
The Zeiss LSM880 Fast Airyscan laser scanning confocal microscope is capable of near-super resolution imaging of commonly used fluorescence dyes on live cells—as well as fixed samples—without the need for special sample preparation techniques or software deconvolution. This inverted microscope is equipped with environmental control and an auto-refocus device for short and long term live cell imaging and fast spectral data acquisition with on-the-fly spectral unmixing, enabling FRET and FRAP to be assessed in real-time and fluorophores/autofluorescence with overlapping emission spectra to be discerned. This instrument features 405, 458, 488, 514, 561 and 633 nm laser lines; a range of dry, water-immersion and oil-immersion objectives; versatility of imaging speeds; very high sensitivity and signal to noise ratios; 2 flanking PMTs, a single-channel GaAsP detector and a transmitted light detector and; DIC optics; set on an antivibration air-table.
Training requirements
In person training to be completed with relevant staff.
Contact person
The VS200 slide scanner is configured for automated imaging of up to 126 slides via fluorescence, brightfield, phase contrast, darkfield and/or polarization microscopy. Various high quality objectives (2x, 4x, 10x, 20x, 40x, 40x oil), fluorescence filters (DAPI, FITC, TRITC, Cy5, Cy7, CFP, YFP, mCherry) and cameras (monochrome and colour CMOS cameras) can be employed. High performance automated ‘count and measure’ and ‘deep learning’ software modules are available and separate data analysis and data storage PC workstations support the VS200 image acquisition system.
In person training to be completed with relevant staff.
The Incucyte SX5 Live-Cell Analysis Instrument is contained within a large (230L) cell culture incubator (37°C, 5%CO2) and enables long-term live cell imaging using high definition phase contrast and up to 3 fluorescence channels in a single experiment (green /orange / near IR) in combination with 4x, 10x or 20x objectives. The system features 3 interchangeable trays that can be used with a large variety of cell culture vessels and up to 6 microplates in parallel. A large number of purpose-built software modules (and relevant accessories) are available for various applications in cell health and proliferation, cell function, 3D cell models, cell movement and morphology (Cell Migration/Invasion, Chemotaxis, Spheroid, Organoid, Cell-by-Cell, Neurotrack, ATP Analysis and Angiogenesis software modules are available.
Training requirements
In person training to be completed with relevant staff.
Contact person
The Cytation 5 combines automated fluorescence and brightfield microscopy and various microplate detection read-outs (fluorescence, luminescence, UV/Vis absorbance, fluorescence polarization and time resolved fluorescence). Imaging can be performed using various plates (including 96- and 384-well plates), slides (including various chamber slides), objectives (4x, 10x, 20x and 40x) and fluorescence filters (DAPI, GFP, Texas Red and Cy5). The dual reagent injector module is also available for use in flash-type luminescence assays and other controlled reagent delivery into microplate wells. Environmental control (temperature and CO2) enables performance of long-term live cell imaging experiments.
In person training to be completed with relevant staff.
The IX83 widefield fluorescence microscope has been customized for sensitive imaging of fluorescently labelled samples. The system features an iXon Life 888 EMCCD camera (Andor) that is configured for ‘SRRF-Stream+’ super-resolution imaging and enables sensitive imaging with fast frame rates and/or low-light illumination of samples. Illumination is provided by a pE-800 LED light engine (CoolLED) equipped with corresponding fluorescent filters (DAPI, CFP, FITC, YFP, TRITC, mCherry and Cy5). A range of objectives are available (10x, 20x, 40x, 60x, 100x) and live cell imaging (37°C, 5%CO2) is supported using a stage-top incubator (Okolab) and an IX3-ZDC2 motorized Z drift compensator.
In person training to be completed with relevant staff.
We have a range of compound and inverted epifluorescence microscopes equipped for camera-based digital image acquisition of slide-mounted samples as well as live cell or organoid cultures in a variety of culture dish types. These are set up to image all common blue, green, red and far-red emitting fluorophores with negligible cross-excitation or emission bleed through. These epifluorescence microscopes enable inexpensive screening and imaging of samples with minimum training required.
In person training to be completed with relevant staff.
We have brightfield compound and stereo microscopes equipped for full colour camera-based digital image acquisition, enabling inexpensive imaging of a variety of samples with minimum training required.
In person training to be completed with relevant staff.
Comprehensive sample preparation expertise and equipment is available to cater for custom preparation of live cells as well as fresh, frozen and fixed tissue samples. We have automated embedding equipment (Leica HistoCore PEARL) and a range of microtomes, sledge microtomes, ultramicrotomes, vibratomes and cryostats available. We can also provide a range of reagents and consumables, including validated secondary antibodies (enzyme and fluorophore conjugates), routine and specialist colourimetric stains, slides, coverslips and coverglass-bottom chambers/dishes. Researchers can opt for sample preparation to be provided or to be trained to access our sample preparation facilities.
In person training to be completed with relevant staff.
All users of our equipment must be trained by our staff. Before booking, please get in touch about your requirements and arrange a time to complete the relevant training. Once you are an authorised and trained user, you may go ahead and book the equipment using the links.
Sturt Rd, Bedford Park
South Australia 5042
South Australia | Northern Territory
Global | Online
CRICOS Provider: 00114A TEQSA Provider ID: PRV12097 TEQSA category: Australian University
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